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Wilfrid Laurier University Faculty of Science
September 2, 2014
 
 
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Instrumentation and Methodology Articles



The following external links highlight new and interesting developments in the fields of instrumentation and methodology.


UV-Vis Spectroscopy

DNA assay by UV: Choose your lab microplates, tubes, and plastic cuvettes carefully! When heated, some polypropylene tubes have been shown to leach chemicals that absorb at 220 and 260 nm, and use of these tubes may skew UV assays for DNA and proteins. The polystyrene microplates used in the study did not show similar leaking characteristics.  
Lewis, L.K. et al. 2010. BioTechniques 48, 297-302 

 
Microscopy

Nanoscopy: Stimulated emission depletion microscopy (STED) has broken the diffraction limit barrier, and enables fluorescent imaging of nanometer-scale structures.

Live Imaging: Single plane illumination microscopes (SPIM) can be used to image live cells for many hours without the photobleaching and phototoxic effects encountered when using fluorescence microscopy. SPIM technology has already been used to capture the embryonic development of zebrafish.

Lens-less: The optofluidic microscope is smaller than a dime, inexpensive to produce, and does not use lenses. It can obtain resolution similar to that of a standard laboratory light microscope. The image is collected when a sample "casts a shadow" on an array of commercial light sensors.

Label-less: Stimulated Raman scattering microscopy (SRS) captures biological molecules without labels or stains by using chemical bond vibrations for imaging.