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Wilfrid Laurier University Faculty of Science
April 17, 2014
 
 
Canadian Excellence

Geoff Horsman



Contact:

email: Geoff Horsman
phone: 519-884-0710
ext: 2985

 

Publications



Horsman GP, Lechner A, Ohnishi Y, Moore BS, Shen B (2013) Predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of nine-membered enediyne antitumor antibiotics. Biochemistry, in press.

Lohman JR, Huang SX, Horsman GP, Dilfer PE, Huang T, Chen Y, Wendt-Pienkowski E, Shen B (2013) Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics. Mol Biosyst, 9:478-491.

Ruzzini AC, Horsman GP, Eltis LD (2012) The catalytic serine of MCP hydrolases is activated differently for C-O bond cleavage than for C-C bond cleavage. Biochemistry, 51:5831-5840.

Lin S, Huang T, Horsman GP, Huang SX, Guo X, Shen B (2012) Specificity of the ester bond-forming condensation enzyme SgcC5 in C-1027 biosynthesis. Org Lett, 14:2300-2303.

Ruzzini AC, Ghosh S, Horsman GP, Foster LJ, Bolin JT, Eltis LD (2012) Identification of an acyl-enzyme intermediate in a meta-cleavage product hydrolase reveals the versatility of the catalytic triad. J Am Chem Soc, 134:4615-4624.

Chen Y, Yin M, Horsman GP, Shen B (2011) Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. J Nat Prod, 74:420-424.

Horsman GP, Chen Y, Thorson JS, Shen B (2010) Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes. Proc Natl Acad Sci USA 107:11331-11335. A news report describing this work appeared in Chem & Eng News (June 14, 2010) 88(24):39.

Ling J, Horsman GP, Huang S-X, Luo Y, Lin S, Shen B (2010) Enediyne antitumor antibiotic maduropeptin biosynthesis featuring a C-methyltransferase that acts on a CoA-tethered aromatic substrate. J Am Chem Soc 132:12534-12536.

Lin S, Horsman GP, Shen B (2010) Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster. Org Lett 12:3816-3819.

Chen Y, Yin M, Horsman GP, Huang S, Shen B (2010) Manipulation of pathway regulation in Streptomyces globisporus for overproduction of the enediyne antitumor antibiotic C-1027. J Antibiot 63:482-485.

Lack NA, Yam KC, Lowe ED, Horsman GP, Nobel R, Sim E, Eltis LD (2010) Characterization of a C-C hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism. J Biol Chem 285:434-443.

Horsman GP, Van Lanen SG, Shen B (2009) Iterative type I polyketide synthases for enediyne core biosynthesis. Methods Enzymol 459:97-112.

Van Lanen SG, Lin S, Horsman GP, Shen B (2009) Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027. FEMS Microbiol Lett 300:237-241.

Lin S, Horsman GP, Chen Y, Li W, Shen B (2009) The SgcF epoxide hydrolase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway generates an (R)-vicinal diol via an enantioconvergent process. J Am Chem Soc 131:16410-16417.

Bhowmik S*, Horsman GP*, Bolin JT, Eltis LD (2007) The molecular basis for inhibition of BphD, a C-C bond hydrolase involved in PCB degradation: large 3-substituents prevent tautomerization. J Biol Chem 282:36377-36385 (*shared first authorship).

Horsman GP*, Bhowmik S*, Seah SYK, Kumar P, Bolin JT, Eltis LD (2007) The tautomeric half-reaction of BphD, a C-C bond hydrolase: kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad. J Biol Chem 282:19894-19904.

Seah SYK, Ke J, Denis G, Horsman GP, Fortin PD, Whiting CJ, Eltis LD (2007) Characterization of a C-C bond hydrolase from Sphingomonas wittichii RW1 with novel specificities towards PCB metabolites. J Bacteriol 189:4038-4045.

Horsman GP, Ke J, Dai S, Seah SYK, Bolin JT, Eltis LD (2006) Kinetic and structural insight into the mechanism of BphD, a C-C bond hydrolase from the biphenyl degradation pathway. Biochemistry 45:11071-11086.

Fortin PD, Horsman GP, Yang HM, Eltis LD (2006) A glutathione S-transferase catalyzes the dehalogenation of inhibitory PCB metabolites. J Bacteriol 188:4424-4430.

Horsman GP, Jirasek A, Vaillancourt FH, Barbosa CJ, Jarzecki AA, Xu C, Mekmouche Y, Spiro TG, Lipscomb JD, Blades MW, Turner RFB, Eltis LD (2005) Spectroscopic studies of the anaerobic enzyme-substrate complex of catechol 1,2-dioxygenase. J Am Chem Soc 127:16882-16891.

Park S, Morley KL, Horsman GP, Holmquist M, Hult K, Kazlauskas RJ (2005) Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations. Chem Biol 12:45-54.

Jirasek A, Horsman GP, Vaillancourt FH, Barbosa CJ, Eltis LD, Blades MW, Turner RFB (2004) Probing enzyme-substrate interactions using fiber-optic ultraviolet resonance Raman spectroscopy. Am Pharm Rev 7:49-53 (Invited Review).

Horsman GP, Liu A, Henke E, Bornscheuer UT, Kazlauskas RJ (2003) Mutations in distant residues moderately increase the enantioselectivity of P. fluorescens esterase towards methyl 3-bromo-2-methylpropanoate and ethyl 3-phenylbutyrate. Chem Eur J 9:1933-1939.

Liu AMF, Somers NA, Kazlauskas RJ, Brush TS, Zocher F, Enzelberger MM, Bornscheuer UT, Horsman GP, Mezzetti A, Schmidt-Dannert C, Schmid RD (2001) Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes. Tetrahedron: Asymmetry 12:545-556.

Beriault JN, Horsman GP, Devine MD (1999) Phloem transport of D,L-glufosinate and acetyl-L-glufosinate in glufosinate-resistant and -susceptible B. napus. Plant Physiol 121:619-627.

Hall LM, Stromme KM, Horsman GP, Devine MD (1998) Resistance to acetolactate synthase inhibitors and quinclorac in a biotype of false cleavers (G. spurium). Weed Sci 46:390-396.