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October 22, 2014
 
 
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Lucy Lee

In vitro cell culture for the study of Loma morhua

Bulletin of the Aquaculture Association of Canada 109-1: 21-27
MacLeod MJ, Rumney R, Monaghan SR, Frenette A, Duffy M, Lee LEJ

published: 2011 | Research publication | Recent Publications

Detailed research into microsporidian parasites using in vivo methodologies is challenging for a variety of reasons. The considerable time and effort needed to secure infective spores, care for livestock, and ensure controlled conditions can substantially slow the pace of research. These challenges are further compounded by lengthy processing procedures and the difficulty in conclusively assigning observations the parasite itself. While not necessarily a replacement for work with whole-organisms, in vitro methods are extremely useful for studies into intracellular pathogens such as Loma morhua, allowing observations of live parasites in situ under controlled conditions. Accordingly, the development of culture methods for Loma and other microsporidian species would facilitate novel research into the life-history, infective processes, and biochemistry of the parasites. To establish laboratory-based culture of Loma morhua specifically, an in vitro infection model has been developed using a novel cod cell line and parasite-specific culture methods. In vitro cultivation of parasites provides a useful model system for assessment of the efficacy of chemotherapeutics that could kill the parasites or limit infection. Infected cell cultures will be treated with a panel of fungicides to identify drugs with potential in treating live cod.

revised Jan 23/12

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