CHARACTERIZING A CELL LINE DERIVED FROM THE BRAIN OF THE AMERICAN EEL, ANGUILLA ROSTRATA
Honours Thesis BSc Thesis
published: 2005 | Thesis | Honours BSc
Nervous tissue tissue-derived cell cultures have proven invaluable tools in neurobiology. They are used to test potential neurotoxicant compounds such as heavy metals, study differentiation of neurons and glia, and to study aspects of neuronal regeneration and senescence. The teleost brain displays extensive adult neurogenesis and post-injury repair in brain tissues, necessitating further study of the cellular mechanisms
behind these novel capabilities. Cell cultures were initiated from brain tissues of an adult American eel ( Anguilla rostrata) in the presence of Lebovitz’s L-15 media supplemented with 20% fetal bovine serum (FBS). A cell line (EelB) has since been established and has retained the diploid chromosome number of the American eel (2n=38) after 19 passages. Cells appear large and epithelioid in nature with highly euchromatic nuclei and numerous lipid-based granules demonstrating yellow-orange autofluorescence.
Immunocytochemical studies have shown reactivity to both anti-glial fibrillary acidic protein (an astrocyte marker) and anti-neurofilament (a neuronal marker), suggesting the cells’ possible identity as neural cell progenitors. A subline of eel brain cells (EelB Sub) which may also be of use to in vitro neurobiology studies has recently been identified, showing rapid proliferation, de-differentiation and a highly aneuploid cell population. The cell lines demonstrate differential responses to toxicants, growth factors, and senescence stains.
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revised May 8/05
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